【摘要】 目的探讨CD4~+CD25~+CD127~-调节性T细胞在慢性丙型肝炎(HCV)患者中的表达及其临床意义。方法收集HCV患者67例,并根据是否吸毒分为吸毒丙肝组35例和非吸毒丙肝组32例;健康体检人员32例作为对照。采用流式细胞仪检测患者外周血中CD~4+CD25~+CD127~-调节性T细胞的表达;应用实时荧光定量PCR的方法检测其HCV RNA含量。结果慢性HCV患者中的吸毒感染组与非吸毒感染组的外周血中CD4+CD25+CD127-调节性T细胞在CD4+T细胞中所占比率分别为:3.84±1.95%、4.44±1.67%,明显低于对照组6.10±1.65%(P<0.01);而吸毒组与非吸毒组慢性HCV患者血液CD4~+CD25~+CD127~-调节性T细胞的表达值相比差异无统计学意义;HCV患者抗病毒治疗前血液CD4~+CD25~+CD127~-调节性T细胞的表达值明显低于治疗后和正常对照组(P<0.01)。结论 HCV患者治疗前患者外周血CD4~+CD25~+CD127~-调节性T细胞的表达值较正常对照组明显降低,而在治疗有效后升高,说明CD4~+CD25~+CD127~-调节性T细胞参与了HCV患者的机体免疫过程,而CD4~+CD25~+CD127~-调节性T细胞在外周血中的表达水平可能成为监测HCV患者体内细胞免疫状态和机体抗病毒免疫应答的指标。更多还原

【Abstract】 Objective To explore the expression of CD4~+CD25~+CD127~-regulatory T Cells from patients with chronic hepatitis C viral infection,and to evaluate its clinical significance. Methods 67 patients with HCV and 32 healthy volunteers were included in this study. The proportion of CD4~+CD25~+CD127~-regulatory T cells in peripheral blood was detected by using flow cytometry. HCV RNA detection in the peripheral blood of HCV patients were done by real- time fluorescent quantitative PCR. Results Comparing with healthy controls,drug abusing and non- drug abusing HCV- infected patients in peripheral blood had a lower percentage of CD4~+CD25~+CD127~-regulatory T cells. The proportion of CD4~+CD25~+CD127~-regulatory T cells to CD4+T cells in peripheral blood from drug abusing and non- drug abusing HCV- infected patients( 4. 44% ± 1. 67%) account for( 3. 84% ± 1. 95%) and( 4. 44% ± 1. 67%) respectively,which was significantly lower than those from healthy controls( 6. 10% ± 1. 65%)( P < 0. 01). However,there was no significant difference between the drug abusing and non- drug abusing HCV- infected patients( P > 0. 05); The expression of CD4~+CD25~+CD127~-regulatory T cells in HCV group before antiviral treatment was evidently lower than that in treated HCV group and control group( P < 0. 01). Conclusion The expression of CD4~+CD25~+CD127~-regulatory T cells in the antiviral treatment HCV patients was evidently higher than those of untreated HCV patients,which explained that the regulatory T cell possibly hold a role in the activation of the immune response to HCV infection. In addition,the expression level of CD4+CD25+CD127-regulatory T cells in peripheral blood may be monitoring the patient’s body cell immune status and become the index of body anti- virus immune response.更多还原