【摘要】 目的探讨甲状腺癌组织中半胱氨酸天冬氨酸蛋白酶(caspase)-9和凋亡抑制蛋白因子(Livin)表达的临床意义。方法检测65例甲状腺癌组织和40例正常甲状腺组织中Caspase-9、Livin表达情况,分别比较Caspase-9、Livin在两种不同组织中表达的阳性率,分析Livin、Caspase-9在甲状腺癌组织中表达情况与临床病理因素的关系,应用Spearman等级相关检验分析Livin、Caspase-9在甲状腺癌组织表达的相关因素。结果 Livin、Caspase-9阳性染色主要位于细胞质内,表现为小片状分布的棕黄色或黄褐色颗粒;甲状腺癌组织中Caspase-9阳性表达率低于正常甲状腺组织(χ2=38.149,P=0.000),Livin阳性表达率高于正常甲状腺组织(χ2=43.161,P=0.000);Livin、Caspase-9在甲状腺癌组织中的表达与患者的年龄、性别及肿瘤的大小、病理类型无关(P>0.05),而与肿瘤的临床分期、淋巴转移和包膜浸润有关(P<0.05);Livin、Caspase-9在甲状腺癌组织中的表达呈负相关(r=-0.346,P<0.05)。结论甲状腺癌组织中Caspase-9表达下调,Livin表达升高,二者的异常表达参与甲状腺癌的发生和发展过程,Livin、Caspase-9联合检测可作为判断甲状腺癌患者预后的重要参考指标。更多还原

【Abstract】 Objective To investigate the clinical significance of expression of cysteine aspartic acid protease( caspase)- 9,Livin in thyroid cancer tissue. Methods The expression of Caspase- 9,Livin of thyroid cancer tissue in 65 patients and normal thyroid tissue in 40 patients were detected. The positive rate of expression of Caspase- 9,Livin were compared in two different kind of tissues. The relationship between the expression of Livin,Caspase- 9 and clinical pathological factors were analyzed with χ2test. The relationship of expression of Livin,Caspase- 9 in thyroid carcinoma tissue were analyzed with Spearman rank correlation test. Results Livin,Caspase- 9 positive staining was mainly located in cytoplasm,and it was showed brown yellow patchy distribution or yellow brown granular. The positive expression rate of Caspase- 9 was lower than that of normal thyroid tissue in thyroid cancer tissue( χ2= 38. 149,P = 0. 000),the positive expression rate of Livin was higher than that of normal thyroid tissue( χ2= 43. 161,P = 0. 000). The expression of Livin,Caspase- 9 in thyroid cancer tissue had nothing to do with the age,gender,tumor size and histologic types of patients( P > 0. 05),but it related to the tumor staging,lymph node metastasis and diolame invasion( P< 0. 05). The expression of Livin,Caspase- 9 in thyroid cancer tissues presented negative correlation( r =- 0. 346,P < 0. 05). Conclusion Expression of Caspase- 9 down- regulated in thyroid cancer tissue,while the expression of Livin increased,the abnormal expression participated in the occurrence and development of thyroid cancer. The combined detection of Livin,Caspase- 9 can be used as an important reference index in judging the prognosis in patients with thyroid carcinoma.更多还原