节点文献

慢性运动性中枢疲劳大鼠额叶皮质单胺类递质变化的研究

Changes of the frontal cortex monoamine transmitters in rats with chronic exercise-induced central fatigue

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 刘占东吴岩珏王得新薛启蓂谢敏豪

【Author】 LIU Zhan-dong;WU Yan-jue;WANG De-xi;Medical Health Center,Beijing Friendship Hospital,Capital Medical University;Centers for Disease Prevention and Control,Atlanta,USA;Department of Neurology,Beijing Friendship Hospital,Capital Medical University;

【机构】 首都医科大学附属北京友谊医院医保中心美国疾病预防与控制中心首都医科大学附属北京友谊医院神经内科北京体育大学

【摘要】 目的研究慢性运动性疲劳状态下,大鼠中枢神经系统各种单胺类递质的变化规律。方法应用慢性运动性中枢疲劳大鼠模型,取材大鼠额叶皮质,采用高效液相色谱电化学检测的方法,对实验大鼠额叶皮质组织5-羟色胺、肾上腺素、去甲肾上腺素及多巴胺的含量进行定量分析。结果与正常饲养大鼠比较,疲劳大鼠在持续训练条件下发生运动性中枢疲劳后,其额叶皮质5-羟色胺含量明显升高,同时多巴胺的含量明显下降,并在统计学出现非常显著性差异(P<0.01);而同样属于单胺类神经递质的肾上腺素和去甲肾上腺素的水平则没有出现统计学差异。结论在中枢性疲劳发生时,中枢神经系统内两种重要神经递质5-羟色胺和多巴胺的水平同时发生了改变,且这两种变化的方向均为抑制性趋势,这也说明该模型大鼠形成了中枢疲劳状态,这种状态的形成引起了脑内递质水平调节机制的变化。

【Abstract】 Objective The paper aims to study the regulatory changes of monoamines transmitters in the central nerve system of rats under the state of chronic exercise- induced fatigue. Methods The rat model of chronic exercise- induced central fatigue was used in this study. The frontal cortex of the rats was removed. The contents of 5- HT,epinephrine,norepinephrine and dopamine in frontal cortex were quantitatively analyzed by high performance liquid chromatography( HPLC). Results It was found that compared with control group,there was a significant increase of frontal cortex 5- HT in rats with exercise- induced central fatigue after constant training and there was,at the same time,a significant decrease of dopamine( P < 0. 01). However,there was no significant change of the levels of epinephrine and norepinephrine which both belong to monoamine neurotransmitter. Conclusion With central fatigue,the levels of 5- HT and dopamine,the two important neurotransmitters in central nerve system,have significant changes simultaneously. And both of the changes have a direct inhibitory trend,which explains that the rats are under the state of central fatigue and this state causes the change of regulatory mechanism of the transmitter in the brain.

【基金】 国家自然科学基金专项基金“中枢性疲劳时有关5-HT能系统的多向性研究:应用生化、电生理、MRS方法对临床病例及实验动物的观察”(SN 81041019);国家科技支撑项目“运动促进体质健康关键技术的研究与应用“分课题”制定有效运动负荷方法与评价等级的研究(SN 2012BAK21B02)”;北京市保健科研课题“慢性失眠对干部人群脑功能影响的研究”(SN京13-03)资助
  • 【文献出处】 临床和实验医学杂志 ,Journal of Clinical and Experimental Medicine , 编辑部邮箱 ,2015年02期
  • 【分类号】R741;R-332
  • 【被引频次】4
  • 【下载频次】171
节点文献中: 

本文链接的文献网络图示:

本文的引文网络