【摘要】 采用溶胶-凝胶法合成TiO₂纳米颗粒,通过TEM对纳米TiO₂进行了表征。结果表明,纳米TiO₂的粒径约为5⁶ mm。利用荧光光谱法研究了纳米TiO₂与肌红蛋白（Mb）的相互作用,研究表明这种相互作用能使肌红蛋白的内源荧光猝灭。通过猝灭常数、结合常数和结合位点数的计算,证明此猝灭为静态猝灭机制,结合位点数约为1。根据热力学参数确定了它们之间的作用力主要是静电力和疏水力,使肌红蛋白构象发生了变化。更多还原

【Abstract】 TiO₂ nanoparticles is synthesized by using the Sol-gel method.The morphology of TiO₂ nanoparticles are characterized by the transmission electron microscopy（TEM）.The interaction between TiO₂ and myoglobin（Mb） is studied by using the fluorescence spectroscopic method.TiO₂ effectively quenches the intrinsic fluorescence of Mb via static quenching.The process of binding TiO₂ on Mb is a spontaneous molecular interaction procedure.According to thermodynamic parameters,△H and AS,it is indicated that the hydrophobic and electrostatic interactions can play a major role in stabilizing the TiO₂-Mb complex.更多还原