【摘要】 采用摇瓶培养的方法,以菌丝生物量和菌丝DNA含量为指标,检查不同浓度的赤霉素(GA)、二氯苯氧乙酸(2,4-D)、玉米素(Zeatin)、萘乙酸(NAA)添加到PDB基质中对刺芹侧耳(Pleurotus eryngii,菌株XH)和金针菇(Flammulina velutipes,菌株G1和F29)菌丝生长的影响。结果表明,在实验设置浓度范围内,适当浓度的GA和2,4-D对三种菌丝生长有正向和负向的明显刺激作用,NAA对三种菌丝的生长有负向强烈刺激作用,Zeatin对菌丝生长的作用不明显。更多还原

【Abstract】 The effects of different concentrations of gibberellin(GA),dichlorophenoxyacetic acid(2,4-D),N,6-isopentenyladenine(zeatin)and naphthylacetic acid(NAA)on the production and DNA content of Pleurotus eryngii(strain XH)and Flammulina velutipes(strains G1 and F29)mycelia grown in submerged culture[100mL potato dextrose broth in 250 mL conical flasks incubated with shaking(120r/min)at 25 ℃for eight days], were determined.Increased mycelia biomass production was observed with some concentrations of GA,2,4-D and zeatin although the effects varied with the fungal strain,whereas NAA was inhibitory.For example,supplementation of cultures with 0.005% GA increased mycelial biomass production in the case of G1 and XH strains(172% and 144%,respectively compared with controls)whereas growth of F29 was unaffected.Conversely,0.001% 2,4-D increased F29 and XH biomass production(164% and154%,respectively compared with controls)but had no effect on the growth of G1.Mycelial DNA content also varied with the level of plant hormone supplementation but no clear patterns were evident.更多还原