A micro spectrophotometer was developed for determination of micro volume solution based on the fiber light source, 50 μL micro volume cuvettea and a minitype-fiber-spectrometer.The performances of this micro spectrophotometer were compared with traditional UV-Vis spectrophotometer by classical spectrophotometry method of determination of iron phenanthroline( phen) spectrophotometry. The results showed that the micro spectrophotometer was consistent with traditional UV-vis spectrophotometer in the precision...