【摘要】 目的探析血清神经生长因子(NGF)水平与肝癌临床病理特征的关系。方法入选的50例原发性肝癌患者作为观察对象,同期体检的50例健康人群作为对照组。采用免疫组织化学(SP)法检测并比较癌组织及癌旁正常组织中NGF的表达,采用酶联吸附免疫(ELISA)法检测2组受试者血清中NGF的浓度,分析其与肝癌临床病理特征的关系。结果癌组织中NGF的阳性表达率为70.0%(35/50),显著高于正常癌旁组织的12.0%(6/50)。观察组血清NGF浓度为(34.0±16.0)pg/ml,显著高于对照组(11.0±6.1)pg/ml。肿瘤直径≥5 cm者NGF浓度为(38.4±18.6)pg/ml,显著高于<5 cm者(26.6±14.3)pg/ml;TNM分期Ⅰ、Ⅱ期者NGF浓度为(25.5±14.6)pg/ml,显著低于Ⅲ、Ⅳ者(39.4±18.7)pg/ml;病理高分化者NGF浓度为(24.8±14.7)pg/ml,显著低于低分化者(40.5±18.8)pg/ml;低侵袭者(25.1±15.0)pg/ml显著低于高侵袭者(39.5±18.5)pg/ml,差异均具有统计学意义(P均<0.05)。结论肝癌患者组织和血清中NGF水平显著升高,且与肿瘤直径大、TNM分期晚、病理分化低及侵袭性高相关。更多还原

【Abstract】 Objective To study the relationship between serum NGF levels and clinical pathological feature of hepatocellular carcinoma. Methods 50 cases of patients with primary liver cancer were the observation group,50 healthy patients were the control group. Immunohistochemical( SP) method was used to detect and compare the expression of NGF in cancer and normal tissues,enzyme-linked immunosorbant assay( ELISA) was used to the serum concentrations of NGF in the 2 groups,its relationship with clinicopathological characteristics of hepatocellular carcinoma were analyzed. Results The positive expression rate of NGF in cancer tissues was 70. 0%( 35 /50),which was significantly higher than that of the normal tissues adjacent to tumor 12. 0%( 6 /50),The serum NGF concentration of the observation group was( 34. 0 ± 16. 0) pg / ml,which was significantly higher than that of the control group( 11. 0 ± 6. 1) pg / ml,NGF level in tumor diameter over 5 cm was( 38. 4 ± 18. 6) pg / ml,which was significantly higher than < 5 cm( 26. 6 ± 14. 3) pg / ml,NGF level in TNM stage Ⅰ and Ⅱ were( 25. 5 ± 14. 6) pg / ml,which were significantly lower than those of the Ⅲ and Ⅳ( 39. 4 ± 18. 7) pg / ml,NGF level in pathology high differentiation was( 24. 8 ± 14. 7) pg / ml,which was significantly lower than low differentiation( 40. 5 ± 18. 8) pg / ml,NGF level in low invasion was( 25. 1 ± 15. 0) pg / ml,which were significantly lower than those of high invasion( 39. 5 ± 18. 5) pg / ml,the difference was statistically significant( P <0. 05). Conclusion The level of NGF in serum and tissues of patients with hepatocellular carcinoma significantly increased,and it is associated with the diameter of tumor,TNM stage,pathological differentiation and high invasion.更多还原