Objective: To study the relationship between S100A2 expressiol in chronic gastritis,intestinal metaplasia,gastric cancer and clinical characteristics. Methods: HE staining was used for gastric specimens histopathological diagnosis. Using immunohistochemistry to detect the expression of tissue S100A2 protein. qRT- PCR was used to detect mRNA expression of S100A2 gene. Western blot for S100A2 gene encoding protein. Kaplan- Meier survival curves were used to distinguish and compare survival. Results: S100A2,S1...
【基金】
辽宁省科技厅基金项目(编号:2013225021)
【更新日期】
2015-10-10
【分类号】
R735.2
【正文快照】
Modern Oncology 2015,23(19):2804-2809后的关系。方法:利用HE染色对所取胃组织标本进行病理组织学诊断;采用免疫组织化学方法检测标本S100A2蛋白的表达;qRT-PCR法检测S100A2 mRNA表达;Western blot检测S100A2蛋白的表达;采用Kap-lan-Meier分析累积生存率。结果:应用qRT-PCR方