【摘要】 目的:探讨氧化苦参碱对人子宫内膜癌细胞HEC-1-B增殖、侵袭转移的作用及其机制。方法:MTT法检测细胞增殖抑制率;Transwell小室法观察细胞侵袭转移的改变;Western blot及Real-time PCR法检测p38、p-p38、MMP-2和MMP-9表达水平的变化。结果:一定浓度氧化苦参碱可显著抑制子宫内膜癌细胞HEC-1-B的增殖、侵袭转移,并呈时间和剂量依赖性。氧化苦参碱可显著降低HEC-1-B细胞中p38及MMPs的表达水平。联合应用SB203580能够有效增强氧化苦参碱的抗肿瘤细胞侵袭转移能力。结论:氧化苦参碱可以抑制子宫内膜癌细胞的增殖、侵袭转移,并下调p38磷酸化以及MMP2、MMP9的表达。更多还原

【Abstract】 Objective:To investigate the effects and mechanisms of oxymatrine on invasion and metastasis of Endometrial carcinoma HEC-1-B cells.Methods:HEC-1-B cells were cultured in vitro and treated by 0,0.5,1.0and 1.5 mg/ml oxymatrine.Then cell growth curves assay and cell invasion assay using Transwell chamber was used to evaluate the effects of oxymatrine on invasion of HEC-1-B cells.The expression of p38,p-p38,matrix metalloproteinases-2(MMP-2)and MMP-9 were analysed by Western blot and Real-time PCR.Results:Compared with control group,oxymatrine(0.5,1.0and 1.5mg/ml)treated groups significantly inhibited the proliferation and invasive ability of HEC-1-B cells in a concentration-and time-dependent manner.In addition,oxymatrine reduced the phosphorylation level of p38 protein and the mRNA levels of MMP-2and MMP-9.Moreover,ap38inhibitorSB203580 and oxymatrine excert synergistic inhibition effects on the expression of MMP-2and MMP-9in HEC-1-B cells.Conclusion:These findings suggest that a potential mechanism by which oxymatrine has an anti-metastatic effects on endometrial cancer cells via directly down-regulating the p38 signaling pathway.更多还原