【摘要】 目的:建立简单、有效的大鼠心肌梗死模型,不用气管插管和呼吸机,直接开胸,在直视下结扎冠状动脉,制备大鼠心肌梗死模型。方法:不用气管插管和呼吸机,经胸骨左缘切口开胸,保持胸膜腔完整,缝扎左室支。术后6周,做病理检查,明确梗死存在并测量梗死面积。结果:大鼠围手术期死亡率23.1%,与假手术组相比,6周后冠脉结扎组左室增大,左室收缩舒张功能受损,病理检查示心肌梗死存在,梗死面积平均为26.6%,两组大鼠心肌梗死面积有显著性差异(P<0.05)。结论:本法成功建立了大鼠心肌梗死模型,具有简便、实用、成功率高之特点;大鼠可作为有关心肌梗死临床研究模型所用合适动物。更多还原

【Abstract】 Objective:To develop a new model of myocardial infarction The rat was not intubated and not ventilated using a animal ventilator.Methods:The rat was not intubated and not ventilated using a animal ventilator.A thoracotomy was performed along left sternum with pleura cavity integrity.The major ventricular branch of the left coronary artery was ligated 1-2mm under the left atria.Sham-operated rats served as controls.Results:Perioperative mortality of rats with myocardial infarction was 23.1%.Six weeks after,echocardiography suggests that the coronary ligation groups had significantly more dilated left ventricles with lower EF、FS than the control group(P<0.05).The infarcted area in the coronary ligation animals was nearly always transmural,the total infarct area ranged from 19 to 38% and there were significant correlations between infarct size and left ventricular impairment as assessed by ejection fraction,left ventricular dimension(P<0.05).Conclusion:We constituted a simple,rapid,and effective method of producing myocardial infarction in rat;rat can be used as a appropriate myocardial infarction model in clinical reaserch.更多还原