【摘要】 目的 探讨胰腺癌中缺氧诱导因子-2α(HIF2α)的表达。并观察HIF-2α的表达对患者预后生存的影响。方法 选取62例胰腺癌标本,采用免疫组织化学方法对胰腺癌组织中的HIF-2α表达进行检测,分析其与临床病理特征及顸后生存的关系。结果 32例胰腺癌组织HIF-2α表达阳性,阳性表达率51.6%(32/62)。10例正常胰腺组织HIF-2α表达均阴性。HIF-2α阳性表达于细胞质。HIF-2α在胰腺癌组织中的表达与肿瘤TNM分期、血管侵犯有关,差异具有统计学意义(X~2=6.452,4.089;P均<0.05),而HIF-2α在胰腺癌组织中的表达与性别、年龄、肿瘤部位、肿瘤大小、淋巴结转移、组织学分级无关,差异无统计学意义(X~2=0.008,0.001,0.518,1.004,2.325,1.617;P均>0.05)。胰腺癌中HIF-2α阴性组病例3年生存率为30%,而HIF-2α阳性组病例3年生存率为6.25%,两组之间差异具有统计学意义(X~2=5.984,P<0.05)。结论 HIF-2α在胰腺癌组织中高表达可能与肿瘤发生、发展密切相关,HIF-2α有望成为胰腺癌新的治疗靶点和预后标志物。更多还原

【Abstract】 Objective To investigate the expression of hypoxia inducible factor alpha-2(HIF-2α) in pancreatic cancer,and to observe the effect of HIF-2α on prognosis.Methods The expression of HIF-2α in 62 pancreatic carcinoma specimen were detected by immunohistochemical method,and their relationships with clinicopathologic characteristics and prognosis of human pancreatic carcinoma were analyzed.Results The positive expression rate in pancreatic carcinoma specimen was51.6%,while there was no positive expression in 10 normal pancreatic specimen.HIF-2α positive expression in cytoplasm.The expression of HIF-2α in pancreatic cancer was associated with TNM staging and vascular invision,the difference was statistically significant(x~2=6.452,4.089;P<0.05),however there was nosignificant correlation between the expression of HIF-2α in pancreatic cancer with sex,age,tumor location,tumor size,lymph node metastasis and histological grade(x~2=0.008,0.001,0.518,1.004,2.325,1.617;P>0.05).3 year survival rate of HIF-2α negative patients was 30%,and that of HIF-2α positive patients was 6.25%,the difference was statistically significant between the two groups(x~2=5.984,P<0.05).Conclusion The high expression of HIF-2αin pancreatic cancer is closely related with carcinogenesis and aggression,and it can be used as a new therapeutic target and prognostic marker of pancreatic carcinoma.更多还原