【摘要】 目的 了解西安地区风疹病毒基因特征,为风疹控制工作提供分子流行病学的基线数据。方法 采集西安地区2013年3起农村学校风疹暴发疫情中27个病例的27份咽拭子标本及6份尿液标本,利用RT-PCR法检测风疹病毒核酸,阳性者接种Vero-SLAM细胞分离病毒,并利用RT-PCR方法鉴定分离到的毒株,对分离到的风疹病毒进行基因序列测定,利用BIOEDIT,MAGA6.0软件进行风疹病毒基因特征分析。结果 27份咽拭子标本中共25份风疹核酸阳性,6份尿液标本均为风疹核酸阳性;从风疹核酸阳性标本分离病毒,25份咽拭子标本和6份尿液标本分别分离到18株和5株风疹病毒株。9株测序成功,经鉴定全部属于1E型基因型。结论 2013年西安地区分离到的风疹病毒株与我国的主要基因型一致,应加强对西安地区风疹血清学诊断、病毒学和血清流行病学监测。更多还原

【Abstract】 Objective To investigate the genetic characteristics of rubella virus strains isolated in Xi’an city,then to provide molecular epidemiological? baseline data for further control.Methods A total of 27 cases of pharyngeal swab specimens and6 cases of urine specimens were collected from rubella outbreaks patients in 3 rubella outbreak in 2013.RT-PCR method was used to detect rubella virus nucleic acid,rubella virus isolated by Vero-SLAM cells and identified by RT-PCR method.The genetic characteristics were analyzed with the virus isolating and RT-PCR method combining.The virus strains were amplified by RT-PCR and sequenced.Phylogenetic tree was constructed by using BIOEDIT and MEGA 4.0 software package.Results Among 27 swab specimens and 6 cases of urine specimens there were 25 and 4 RUV RNA positive.18 strains of rubella virus strain isolated from 23 RUV RNA positive(swab specimens) and 5 strains from 6(urine specimens) specimens in xi’an region,but only 9 rubella virus strains via sequencing appraisal all belong to genotype 1E genotype.Conclusion 1E genotype of rubella virus is the predominance genotype in Xi’an city.Should strengthen the rubella serological diagnosis,virology and serum epidemiological surveillance.更多还原