【摘要】 目的 评估Beckman Coulter实验室自动化系统生化免疫样品间携带污染情况,探讨不选择分杯模块时生化免疫共用一管血的可行性。方法 将高浓度及不同样品量(2 000μl,500μl)低浓度的HCG样品按照一定顺序在Becκman AU5421全自动生化分析仪上检测生化项目,比较检测生化项目前后低浓度样品HCG含量的变化评估生化免疫样品间携带污染情况,并在增加Beckman AU5421全自动生化分析仪特殊清洗程序后重复试验。结果 在不同样品量(2 000μl,500μ1)条件下,Becκman AU5421全自动生化分析仪单模块及整机携带污染分别为5.44,15.47,23.51,45.96 ppm,不同样品量及单模块与整机间携带污染差异均有统计学意义(t=14.553,P<0.001;t=5.527,P=0.005;t=3.985,P=0.016;t=20.457,P<0.001);增加Beckman AU5421特殊清洗程序后仅检出500μl样品量时整机携带污染为0.22 ppm。结论不选择分杯模块时,Becκman Coulter实验室自动化系统可使用特殊清洗程序解决生化免疫间携带污染。更多还原

【Abstract】 Objective To evaluate the Carryover between the chemistry and immunoassay in Beckman Coulter Laboratory Automation System and decide to whether sharing samples for testing between chemistry and immunoassay systems or not.Methods According to a certain order,high concentration samples and low concentration samples of HCG with different sample volume(500 μl,2 000 μl) were tested on Beckman AU5421 automatic biochemical analyzer.The HCG of low concentration samples were then tested to evaluate the carryover between the chemistry and immunoassay and explored the corrective procedure to deal with the carryover by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.Results Under different sample volume,the carryover in a single module and as a whole of the beckman AU5421 automatic biochemistry analyzer were 5.44,15.47,23.51 and 45.96 ppm respectively(t=14.553,P<0.001;t= 5.527,P =0.005;t=3.985,P = 0.016;t=20.457,P<0.001).By increasing special cleaning process the carryover of 0.22 ppm was detected in 500 μl sample volume of the beckman AU5421 automatic biochemistry analyzer as a whole.Conclusion The carryover between the chemistry and immunoassay in Beckman Coulter Laboratory Automation System could been sovled by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.更多还原