【摘要】 目的 探讨Bcl-6 mRNA在HIV/AIDS患者滤泡辅助性T细胞(Tfh)中的表达及-其与疾病进展的关系。方法 筛选2014年5月~11月期间河南中医学院第一附属医院艾滋病临床研究中心经HIV抗体确证实验阳性者60例,根据CD4~+T细胞数将HIV AIDS患者分为A(CD4<200个/μl),B(200个/μl<CD4<500个/μl)和C(CD4>500个/μl)三组,同期选取20例健康对照组,采用流式细胞术(FCM)检测淋巴细胞亚群(T,B,NK细胞)和Tfh细胞(CD4~+CXCR5~+ICOS~+)的百分比。实时荧光定量PCR技术检测Bcl-6 mRNA的表达,并分析Tfh细胞与其转录因子Bcl 6 mRNA的相关性,Tfh细胞与B细胞的相关性。结果 A,B和C三组Bcl-6 mRNA表达均高于健康对照组(2.94±0.91,2.27±0.62,2.15±0.351,P值均<0.05);A组Tfh细胞大于C组(5.88±3.01 vs 1.2640.87,P=0.032)和健康对照组(5.88±3.01vs 0.78±0.42,P=0.004);四组NK细胞和B细胞比较差异无统计学意义(P=0.364,0.571);Tfh细胞与Bcl-6 mRNA正相关(r=0.799,P=0.000),Tfh细胞与B细胞无关联(r=-0.083,P=0.657)。结论 Bcl-6 mRNA和Tfh细胞的变化可能与HIV/AIDS疾病进展有关。更多还原

【Abstract】 Objective To explore the expression of Bcl-6 mRNA in the Tfh cells of HIV/AIDS patients and the relationship between Bcl-6 mRNA and the progression of HIV/AIDS.Methods This experiment chose 60 patients who were confirmed by HIV antibody test positive,during May 2014 to November from AIDS Research Institute in the First Affiliated Hospital,Henan College of Traditional Chinese Medicine.According to the amount of CD4 + T cells,the patients with HIV/AIDS were divided into A(CD4<200 cells/μl),B(200 cells/μl<CD4<500 cells/μl) and C(CD4>500 cells/μl) three groups.The Tfh cells expressed CD4~+CXCR5~+ICOS~+ and lymphocyte subpopulation including T,B and NK cells were detected by flow cytometry(FCM).The expression of Bcl-6 mRNA levels were detected by reverse transcription-polymerase chain reaction(RT-PCR).The association between Tfh cells and Bcl-6 mRNA,B cells were analyzed.Results The expression of Bcl-6mRNA from patients of A,B and C three groups was increased than in healthy individuals(2.94±0.91,2.27±0.62,2.15±0.351,P<0.05).The percent of Tfh cells from group A patients was higher than group C patients(5.88±3.01 vs 1.26±0.87,P = 0.032) and also the healthy control group(5.88±3.01 vs 0.78±0.42,P = 0.004).The percentage of NK cells and B cells in HIV/AIDS patients was no statistically significant.Correlation analysis showed that there was positive correlation between the proportion of Tfh cells and Bcl-6 mRNA(r=0.799,P — 0.000).And Tfh cells were observed no relationship with B cells(r= —0.083,P = 0.657).Conclusion The changes of Bcl-6 mRNA and Tfh cells may be related to the progression of HIV/AIDS.更多还原