【摘要】 目的评价三七的止血作用。方法采用肝脏局部创伤的方法复制小鼠肝脏局部创伤出血模型,实验分为空白组、羧甲基纤维素钠组、淀粉组、云南白药组和三七粉组,各组局部给药,观察药物对出血量的影响;灌胃给予大鼠三七粉,连续8 d后,静脉注射肝素300 U/kg,15 min后,参照小鼠方法复制大鼠肝脏局部创伤出血模型,测定出血量;灌胃给予大鼠三七粉等药物,连续8 d,舌下静脉注射肝素造成肝素化大鼠模型,测其活化部分凝血酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)。结果三七粉局部给药能减少小鼠肝脏局部创伤出血模型的出血量(P<0.05),但与羧甲基纤维素钠组、淀粉组、云南白药组比较无明显差异,提示三七粉局部给药能减少出血量的作用,可能与其细粉状态有关,属非特异性止血。三七粉灌胃能减少肝素化大鼠肝脏局部创伤模型的出血量(P<0.05),抑制率为16.1%,表明止血作用较弱。三七粉灌胃能缩短肝素化大鼠APTT、TT(P<0.05),抑制率分别为25.7%、18.8%,对PT无影响。提示,三七有止血作用,可能与干预内源性凝血途径,促纤维蛋白形成有关。结论三七粉体内外均有止血作用,其中体外止血可能是物理性、非特异性的,体内止血可能与其部分对抗肝素的抗凝作用有关。更多还原

【Abstract】 Objective: To evaluate the hemostasis function of Sanqi. Methods: Established by duplicated rats with hepatic topical trauma,the bleeding models were divided into blank,sodium carboxymethyl cellulose( CMC),starch,Yunnan Baiyao and San Qi powder groups. The medicines were applied topically for each group to observe their effects in bleeding; the San Qi powder was intragastrically administered to rats for 8 days,which were intravenously injected 300 U /kg heparin and duplicated the bleeding models of hepatic topical trauma consulted the mice methods after 15 minutes to determine the bleeding volume; the San Qi powder and other drugs were intragastrically administered to rats for 8 days,which intravenously injected heparin under the tongue to make the heparin rats models to examine the activated partial thromboplastin time( APTT),prothrombin time( PT) and thrombin time( TT). Results: The topical administration of San Qi powder could reduce the bleeding of mouse hepatic topical bleeding models( P < 0. 05),but there was no significant difference in the comparison of all the groups,which indicated that the effect of San Qi powder in reducing small amount of bleeding volume might be due to its fine powder,belonging to the unspecific hemostasis. The intragastric administration of San Qi powder might decrease the bleeding volume of the heparin rats models( P < 0. 05) with the inhibition rate of 16. 1 %,which indicated that its hemostasis function was lower. The intragastric administration of San Qi powder could shorten the APTT and TT of the heparin rats models( P < 0. 05) with their inhibition rates of 25. 7% and 18. 8%respectively,but there was no influence over the TT. The results indicated that Sanqi had the function of stopping bleeding which might have something with its interference of the intrinsic pathway and promotion of the fibrin formation. Conclusion: SanQ i powder administered orally and topically has the hemostasis function and its hemostasis function outside the body may be physiological and unspecific while inside the body may be related to its partial function of anticoagulation to fight against heparin.更多还原