Objective To evaluate the linear detection range, accuracy and minimum detection limit of seven reagents for HBs Ag of three subtypes. Methods High concentration HBs Ag samples were quantitatively demarcated by parallel line end dilution method using HBs Ag reference standard provided by World Health Organization(WHO) to evaluate the effective detection range of the reagent. The accuracy of reagent for quantitation was evaluated by using the samples at concentrations of 5. 9 ~ 100 IU / ml. HBs Ag samples of...
【基金】
“十二五”国家科技重大专项(2012ZX10004-702)
【更新日期】
2016-01-05
【分类号】
R446.6
【正文快照】
乙型肝炎病毒表面抗原(hepatitis B surface antigen,HBs Ag)是乙型肝炎病毒感染的重要标志[1]。临床上HBs Ag定量检测已广泛用于抗病毒药物治疗疗效观察及愈合评价[2-3]。HBs Ag定量检测方法是利用双抗体夹心的原理,应用多个单克隆抗体和多克隆抗体进行组合,检测样品中的HBs A
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