目的比较灭能与未灭能新生牛血清培养Vero细胞和MRC-5细胞的效果,为细胞培养的相关研究和生产提供参考。方法分别采用照射剂量为8和16 k Gy的60Coγ-射线及56℃30 min加热灭能新生牛血清,用两种方式灭能的血清及未灭能的新生牛血清培养Vero细胞和MRC-5细胞,显微镜观察细胞的生长情况,计数并计算细胞倍增时间及存活时间。结果未灭能新生牛血清培养的两种细胞增殖更多更快速;热灭能的新生牛血清培养的两种细胞的增殖速度和数量明显低于γ-射线照射灭能及未灭能血清培养的细胞。结论未灭能的新生牛血清比灭能后的血清培养Vero细胞和MRC-5细胞的效果好,其促细胞生长增殖效果最佳。
【英文摘要】
Objective To compare the effects of inactivated and active newborn calf sera(NCS)in cultures of Vero and MRC-5 cells and provide a reference for study and production of cell culture. Methods NCS was inactivated by radiation with 8 and 16 k Gy60 Co γ-ray and heating at 56 ℃ for 30 min respectively. Vero and MRC-5 cells were cultured by the NCS inactivated by two methods as well as active NCS, observed for growth by microscopy and counted, of which the doubling and survival times were calculated. Results Comp...
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