Objective To prepare human LIGHT-Fc fusion protein by using yeast expression system. Methods The recombinant plasmid p PIC9K-LIGHT-Fc containing the gene of outer membrane domain of human LIGHT and human Ig G4 Fc was constructed by genetic engineering technology, linearized with Sal Ⅰ and electrotransformed to competent yeast GS115. The recombinant transformants were identified by PCR, of which the positive ones were induced with methanol. The transcription level of target gene was determined by RT-PCR, whi...
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