【摘要】 目的:在体外培养大鼠前列腺上皮细胞,建立脂多糖和蛇毒诱导的大鼠前列腺上皮细胞的体外炎症模型。方法:采用WAJC404培养基在体外培养大鼠的前列腺上皮细胞,通过免疫细胞化学方法对其进行鉴定,加入脂多糖和蛇毒用以建立体外的前列腺炎症模型。结果:在体外培养了大鼠的前列腺上皮细胞,泛细胞角蛋白染色阳性,脂多糖和蛇毒可损伤体外培养的前列腺上皮细胞,但不影响体外培养的前列腺上皮细胞培养液中细胞因子TNF-α和IL-1β及免疫球蛋白IgG、IgA和IgM的含量。结论:在体外培养了大鼠的前列腺上皮细胞,对大鼠前列腺体外炎症模型的建立进行了初步探索。更多还原

【Abstract】 Objective: To culture the rat prostate epithelial cells in vitro,and establish the prostatitis model withlipopolysaccharides and venin.Methods: We use the WAJC404 culture medium to culture the rat prostate epitheli-al cells,and identify the cells with the immunocytochemistrymethod.We also add lipopolysaccharides and venin inthe medium to establish the prostatitis model.Results: We get the rat prostate epithelial cells sussessfully and getthe positive result by pan-cytokeratin staining.Lipopolysaccharides and venin can injure the cells but not changethe level of cytokines TNF-α,IL-1β and aslo immunoglobulins IgG,IgA and IgM in the culture medium.Conclusion: We cultured the rat prostate epithelial cells in vitro sussefully and did preliminary study of the prostatitismodel establishment in vitro.更多还原