【摘要】 目的探索以胶原、透明质酸钠和硫酸软骨素三种细胞外基质为原料,利用静电纺丝技术构建组织工程关节软骨支架的工艺。方法固定静电纺丝的参数,通过改变溶剂的种类,浓度和三种原料之间的比例,探索胶原-透明质酸钠-硫酸软骨素复合支架的纺丝条件。结果胶原-透明质酸钠-硫酸软骨素复合支架纺丝的最佳溶剂是3-氟乙醇和水的混合溶剂(v:v,1:1),最佳纺丝浓度范围为80~120mg/m L,三种原料支架的最佳比例范围为6~8:1:1~2。支架纤维的直径和孔隙率则由原料的浓度和比例共同决定;支架降解性能良好,降解速度主要由胶原含量决定。结论研究者成功的利用静电纺丝技术构建了胶原-透明质酸钠-硫酸软骨素复合组织工程软骨支架。这种支架在成分和形态上都可以较好的模拟关节软骨细胞外环境,是一种具有良好应用前景的组织工程关节软骨支架。更多还原

【Abstract】 Obejective To explore the process of constructing tissue-engineered articular cartilage scaffold with extracellular matrix-collagen, hyaluronic acid and chondroitin sulfate by electrospun. Methods Electrospun parameters fixed,the solvent, concentration and the ratio among the materials were changed to explore the conditions of constructing scaffold. Results The best solvent of electrospinning was 3-Trifluoroethanol mixed with water(v : v, 1 : 1); the optimum concentration range was 80 to 120mg/m L and the optimum ratio range among the materials was 6 to 8 : 1 : 1 to 2. Fiber diameter and porosity were decided by the concentration and the ratio of the materials. The degradation of the scaffold was good, which was mainly decided by the content of the collagen. Conclusion The collagen-hyaluronic acid-chondroitin sulfate scaffold was built by electrospun for tissue-engineered articular cartilage. This scaffold can simulate the composition and the morphology of articular cartilage, which has a good prospect on tissue-engineered articular cartilage.更多还原