【摘要】 对烟草节杆菌发酵产酶培养基进行了优化。在烟草节杆菌的初始发酵培养基条件下,肌酐酶初始酶活仅为9.2U/g湿菌。首先,通过肌酸诱导、金属离子筛选实验发现肌酸和金属离子对烟草节杆菌产肌酐酶酶活有重要影响;然后再通过碳源和氮源优化,以玉米浆和酵母膏作为复合氮源,可溶性淀粉为碳源,肌酐酶产量可达到108.5 U/g湿菌;在以上基础上,最后通过两次正交试验优化初始发酵培养基,最优培养基组成为:肌酸0.3%,玉米浆0.3%,可溶性淀粉0.4%,酵母膏0.7%,Fe²+0.003%,Mn²+0.006%,Mg²+0.005%,K₂HPO₄0.1%,KCl 0.5%。在优化后的发酵培养基条件下,烟草节杆菌肌酐酶酶活达到182.82 U/g湿菌,为初始发酵培养基酶活的19.87倍。更多还原

【Abstract】 The optimal culture medium of creatininase production on the Arthrobacter nicotiana were carried out. Firstly,using creatine as inductor and metal ions experiment,it was found that creatine and metal ions have a great influence upon the creatininase production. Secondly,single factor experiment showed the optimization carbon and nitrogen sources was soluble starch and corn steep liquor,but compound nitrogen source of yeast extract and corn steep liquor was much better( creatininase production reached 108. 5 U / g wet mycelium). At last,creatininase production reached 182. 5 U / g wet mycelium under the optimization of fermentation medium,which was about 19. 87 times as high as the initial fermentation enzyme activity.更多还原