【摘要】 目的:研究"促育生精方"对精子特异性钙通道蛋白Cat Sper1、Cat Sper2表达的影响。方法:采用Real-time PCR法检测Cat Sper1 m RNA、Cat Sper2 m RNA在各组大鼠(模型组、低剂量组、中剂量组、高剂量组、空白对照组)精子中的表达;用Western blot检测各组Cat Sper1蛋白,Cat Sper2蛋白的表达。结果:成功建立大鼠不育模型。Cat Sper1 m RNA、Cat Sper2 m RNA表达量为:中、高剂量组显著高于模型组(P<0.05)。Cat Sper1、Cat Sper2蛋白表达量:中、高剂量组显著高于模型组(P<0.05)。结论:促育生精方能有效提高少弱精症模型大鼠精子特异性钙通道Cat Sper1、Cat Sper2基因及其蛋白的表达。更多还原

【Abstract】 Objective: To investigate the effect of "Cuyushengjingfang" on the expression of Cat Sper1 and Cat Sper2 in the sperm of Oligoasthenozoospermia Wistar rats induced by Cyclophosphamide(CP). Methods: Thirty male Wistar rats were randomly divided into model group(MG), low-dose Cuyushengjing group(LDCG), middle-dose Cuyushengjing group(MDCG), high-dose Cuyushengjing group(HDCG) and blank control group(BCG). Model rats of oligoasthenozoospermia were created by CP(30 mg/kg) injected into the abdominal cavity of rats once a day for 5days, while BCG were injected with normal saline(NS). The rats in LDCG, MDCG and HDCG were oral administrated with 3ml Cuyushengjingfang for 35 days while the rats in MG and BCG were oral administrated with 3ml NS. The concentration of Cuyushengjingfang in that of LDCG, MDCG and HDCG were 0.84 g/ml, 1.69 g/ml, and 3.37 g/ml. The proteins expression of Cat Sper1 and Cat Sper2 in sperm was measured by Western blotting and Real-time PCR. Results: Models rats of Oligoasthenozoospermia were created successfully. The m RNA expression of Cat Sper1 and Cat Sper2 in MDCG and HDCG were significantly higher than that in MG(P<0.05). The protein expression of Cat Sper1 and Cat Sper2 in MDCG and HDCG were higher than that in MG(P<0.05). Conclusion: Cuyushengjingfang could effectively increase the expression of Cat Sper1 and Cat Sper2 in model Rats of Oligoasthenozoospermia created by Cyclophosphamide.更多还原