In this study, the mature peptide-encoding c DNA sequence of the growth hormone of Pelteobagrus fulvidraco(Pf GH) was inserted into the prokaryote expression vector p ET-28a(+). The recombinant plasmid of p ET-Pf GH was then transfected into Escherichia coli BL21(DE3) and was induced by IPTG. The SDS-PAGE results showed that E. coli BL21(DE3) with p ET-Pf GH expressed a specific fusion protein with a molecular weight of 21 KD. The western blot test revealed that the protein contained a 6-His tag. Pf GH was ...
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