【摘要】 目的检测信号转导与转录激活因子6(signal transducers and activators of transcription 6,STAT6)在正常食管黏膜、癌旁非典型增生和食管鳞癌组织中的表达,探讨其与食管鳞癌临床病理参数的关系。方法 2013年8月—2014年5月应用RT-PCR和Western Blotting方法检测40例食管鳞癌、40例癌旁非典型增生及20例正常食管黏膜组织中STAT6的表达。多组间比较用单因素方差分析,多样本率的比较用行列表资料的χ2检验,P<0.05为差异有统计学意义。结果 STAT6 m RNA在正常管黏膜组织、非典型增生组织和食管鳞癌组中都有表达,其相对表达强度依次升高,分别为(0.085±0.000)、(0.519±0.001)、(0.860±0.000),组间差异有统计学意义(F=2.030,P<0.05);STAT6蛋白在正常食管黏膜组织、非典型增生组织中及食管鳞癌组织中的表达量依次升高,分别为(0.339±0.001)、(0.486±0.001)、(0.624±0.001),组间差异有统计学意义(F=894.735,P<0.05);STAT6异常表达与食管癌的分级、浸润深度及淋巴结转移均密切相关(P<0.05)。结论 STAT6的表达增强可能与食管鳞癌的发生发展有关。更多还原

【Abstract】 Objective To detect expression of signal transducers and activators of transcription 6(STAT6) in 20 cases of normal esophageal tissues,40 cases of dysplasia and 40 cases of esophageal squamous cell carcinoma and to explore its correlation with clinicopathologic characteristics. Methods The expression levels of STAT6 protein and m RNA were detected in20 cases of normal esophageal tissues,40 cases of dysplasia and 40 cases of esophageal squamous cell carcinoma by RT- PCR and Western Blotting. Using SPSS17.0 software for processing,compared with single factor analysis of variance between groups,variety of this rate compared with chi- square test table data,the result of P<0.05 was considerell statisticaly significant. Result Positive expression rates of STAT6 m RNA in the normal esophageal tissues,atypical hyperplasia and squamous carcinoma of the esophagus were expressed in the group,increase the intensity of the relative expression,respectively(0.085±0.000)、(0.519±0.001)、(0.860±0.000),the difference between groups was statistically significant(F=2.030,P <0.05).Positive expression rates of STAT6 protein in the normal esophageal tissues,atypical hyperplasia and squamous carcinoma of the esophagus were expressed in the group,increase the intensity of the relative expression,respectively(0.339±0.001)、(0.486±0.001)、(0.624±0.001),the difference between groups was statistically significant(F=894.735,P <0.05).The abnormality expression of STAT6 were positively correlated with tumor grade,infiltration and lymphatic metastasis of esophageal squamous cell carcinoma tissue(P<0.05). Conclusion Enhancement expression of STAT6 may be related to the occurrence and development of esophageal squamous cell carcinoma.更多还原