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响应面法优化蛇足石杉中石杉碱甲的提取工艺

Optimization of Extraction Process for Huperzine A from Huperzia serrata

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【作者】 杨婷武芸张泽武玉莲郑小江胡世坤毛鑫

【Author】 YANG Ting;WU Yun;ZHANG Ze;WU Yulian;ZHENG Xiaojiang;HU Shikun;MAO Xin;Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province,Hubei Institute for Nationalities;College of Biological Scientific and Technical, Hubei University for Nationalities;College of Life Sciences, Sichuan University;University Hospital of Hubei University for Nationalities;

【机构】 湖北民族学院生物资源保护与利用湖北省重点实验室湖北民族学院生物科学与技术学院四川大学生命科学学院湖北民族学院附属民大医院

【摘要】 利用响应面分析法优化恩施州蛇足石杉中石杉碱甲的提取工艺条件。在以提取温度、提取时间、料液比、酒石酸质量分数为影响因素,以石杉碱甲提取量为指标的单因素试验基础上,根据Box-Behnken原理设计三因素三水平的试验分析法,得到提取石杉碱甲最佳工艺条件:料液比1∶30(g/m L)、酒石酸质量分数1.5%、提取温度41℃。采用高效液相色谱法检测石杉碱甲含量,在最优提取条件下,热水浸提4 h,石杉碱甲提取量为11.192 8 mg/100 g。

【Abstract】 This paper is concerned with the selection and optimization of important extraction parameters as well as their levels for the increased yield of huperzine A from the aboveground parts of Huperzia serrata by combined use of single factor method and response surface methodology(RSM). Solid-to-solvent ratio, solvent concentration and temperature were identified as main variables that affect extraction efficiency, and their levels were optimized using RSM based on a threevariable, three-level Box-Behnken design. When the extraction was performed for 4 h under the optimized conditions: 1.5%tartaric acid as extraction solvent with a solid-to-solvent ratio of 1:30(g/m L) and an extraction temperature of 41 ℃, the yield of huperzine A was 11.192 8 mg/100 g.

【基金】 湖北省教育厅科学技术研究项目(D20141903);湖北省自然科学基金项目(2014CFB615);生物资源保护与利用湖北省重点实验室开放基金项目(PKLHB1301);恩施州2013科技研究与开发项目(2013002);国家级大学生创新课题项目(201210517030);湖北民族学院大学生创新训练计划项目(2014Z064);湖北民族学院林学一级学科(0907)资助项目
  • 【分类号】TQ283
  • 【被引频次】4
  • 【下载频次】372
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