【摘要】 利用响应面分析法优化恩施州蛇足石杉中石杉碱甲的提取工艺条件。在以提取温度、提取时间、料液比、酒石酸质量分数为影响因素,以石杉碱甲提取量为指标的单因素试验基础上,根据Box-Behnken原理设计三因素三水平的试验分析法,得到提取石杉碱甲最佳工艺条件:料液比1∶30(g/m L)、酒石酸质量分数1.5%、提取温度41℃。采用高效液相色谱法检测石杉碱甲含量,在最优提取条件下,热水浸提4 h,石杉碱甲提取量为11.192 8 mg/100 g。更多还原

【Abstract】 This paper is concerned with the selection and optimization of important extraction parameters as well as their levels for the increased yield of huperzine A from the aboveground parts of Huperzia serrata by combined use of single factor method and response surface methodology(RSM). Solid-to-solvent ratio, solvent concentration and temperature were identified as main variables that affect extraction efficiency, and their levels were optimized using RSM based on a threevariable, three-level Box-Behnken design. When the extraction was performed for 4 h under the optimized conditions: 1.5%tartaric acid as extraction solvent with a solid-to-solvent ratio of 1:30(g/m L) and an extraction temperature of 41 ℃, the yield of huperzine A was 11.192 8 mg/100 g.更多还原