Glutamate decarboxylase( GAD) is a key enzyme involved in the biosynthesis of γ- aminobutyric acid.In this study,a recombinant Bacillus subtilis expressing glutamate decarboxylase was constructed through genetic engineering,and fermentation conditions for production of GAD were optimized by single- factor test,orthogonal test and response surface methodology,and the conditions were as follows,sucrose 23.5,soybean meal 10,ammonium acetate 8.5,potassium dihydrogen phosphate( KH2PO4) 4.1,magnesium sulfate hept...
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