The process of bed reactor with immobilized inulin fructotransferase for inulin hydrolysis was founded.The conditions of inulin hydrolysis were explored. Based on single factors experiments,orthogonal experiment was adopted to investigate the influences of inulin concentration,reaction time,reaction temperature and substrate velocity on DFA Ⅲ contents. The optimal conditions were obtained. This process was adopted with the volume of packed bed reactor for 20 m L and loading amount of immobilized enzyme for ...