Objective:To compare the content of Saponins in Gynostemma pentaphyllum(Thunb.) Makino from different regions. Methods :The HPLC separation was carried out with a C18column(4.6mm ×250mm,5μm)using acetonitrile and water as mobile phase at a flow rate of 0.8m L·min-1. The detection wavelength was203 nm and the column temperature was maintained at 25℃. Results:The material collected from Fengyukou in Shaanxi had the highest content of ginsenoside Rb1,Rb3 and Rd,about 1.625%,1.776%,0.892% respectively.The mater...