【摘要】 以枇杷籽为原料,研究枇杷籽超氧化物歧化酶的提取及纯化工艺。研究结果表明:枇杷籽超氧化物歧化酶提取最佳工艺条件为:超声功率为295.4 W,提取时间为22.8 min,p H为6.7,液料比为20.8∶1(m L/g)。枇杷籽超氧化物歧化酶分离纯化工艺为:依次经过热变性法处理、丙酮沉淀、G-75葡聚糖凝胶过滤,得到枇杷籽超氧化物歧化酶的蛋白去除率为92.1%,总酶活为1 091.6 U,酶比活力为4 746.1 U/mg,纯化倍数为56.5。更多还原

【Abstract】 Mainly discuss the extraction technology and purification technology of superoxide dismutase from Eriobotrya japonica seed.The optimum extraction conditions of superoxide dismutase from Eriobotrya japonica seed were determined,such as extraction power for 295.4 W,ultrasonic extraction time for 22.8 min,pH for 6.7,and the solution and material ratio for 20.8:1(mL/g).The optimum purification conditions of superoxide dismutase from Eriobotrya japonica seed were also determined.It is purified by thermal denaturation,acetone sediment and Sephadex G-75 column chromatography,respectively.The result of superoxide dismutase from Eriobotrya japonica seed through purification is the removal rate of protein for 92.1%,total enzyme activity for 1 091.6 U,the specific activity of enzyme for 4 746.1 U/mg,and the purify multiple for 56.5.更多还原