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响应面法优化杨梅叶中黄酮提取工艺的研究

Extracting Total Flavonoids from Myrica rubra Leaves Determined by Using Response Surface Methodology

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【作者】 李尚谦张斌郭辉钱俊青

【Author】 Li Shang-qian;Zhang Bin;Guo Hui;Qian Jun-qing;College of Pharmaceutical Science, Zhejiang University of Technology;

【机构】 浙江工业大学药学院

【摘要】 杨梅叶中的黄酮类活性成分具有抑制血小板聚集、调血脂、抗氧化、抑菌、抗肿瘤等作用。使用乙醇浸体法提取杨梅叶中的黄酮类活性成分,并采用响应面法优化提取工艺条件。最终得到最佳提取条件为料液比1∶64(g/m L),提取温度52.5℃,乙醇体积分数为64%,提取时间为4.5 h,杨梅黄酮提取率达到4.01 mg/g。

【Abstract】 To de termine the proper factor for extracting total flavonoids from Myrica rubra leaves, several factors, such as the temperature, concentration of extraction solvent, time, and the solid/liquid ratios, were investigated and the optimal conditions were obtained from the experiments with response surface methodology. The optimum condition for extracting total flavonoids was in 64% ethanol at 52.5 ℃ for 4.5 h and the solid/liquid ratios was 1∶64(g/m L). The extraction percentage of flavonoids was 4.01 mg/g with the described conditions.

【关键词】 杨梅黄酮提取响应面
【Key words】 Myrica rubraflavonoidsextractorthogonal experiments
【基金】 浙江省创新团队项目(2010R50032)
  • 【分类号】TQ464
  • 【被引频次】6
  • 【下载频次】202
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