Auto-induced expression of Streptomyces griseochromogenes trehalose synthase gene in E. coli and optimization of its two-stage temperature controlling fermentation
A novel trehalose synthase gene was cloned from Streptomyces griseochromogenes YX-1 and overexpressed in Escherichia coli BL21( DE3),and the auto-induced fermentative condition of recombinant bacteria was further optimized. Trehalose synthase gene was successfully amplified from S. griseochromogenes by degenerate PCR. The amplified fragment was linked to p ET28 a to construct p ET28a-tres which were then transformed into E. coli BL21( DE3). The recon was screened and gene was expressed by auto-induced ferme...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
