【摘要】 目的提高茶叶中粗多糖的提取率。方法以汉中红茶为原料,采用复合酶(纤维素酶、果胶酶)法提取粗多糖。在单因素试验的基础上,通过L9(34)正交试验优化了复合酶添加量,提取温度,p H值和料液比等工艺参数。结果影响粗多糖得率的因素依次为提取温度(B)>复合酶添加量(A)>p H值(C)>料液比(D),最佳提取工艺参数为复合酶(果胶酶:纤维素酶=2:1)添加量0.8%,提取温度50℃,p H值4.0,料液比1:25。在此条件下粗多糖的平均得率为5.97%,是水提法的2.61倍。结论该研究优化的工艺参数具有提取效率高、提取温度低的特点。更多还原

【Abstract】 Objective To improve the extraction rate of the coarse polysaccharides. Methods Coarse polysaccharides were extracted from Hanzhong black tea by using composite enzymes(cellulose enzyme and pectinase). Based on the single factor experiments, the optimum conditions of the amount of composite enzymes, the extracting temperature, the p H and the solid-liquid ratio were optimized by orthogonal design L9(34). Results The impact factors were as follows: the extracting temperature(B)>the amount of composite enzymes(A)>the p H(C)>the solid-liquid ratio(D). The optimized process parameters were the amount of composite enzymes 0.8%, the extracting temperature 50 ℃, the p H 4.0, the solid-liquid ratio 1:25. Under the above conditions, the extraction rate of coarse polysaccharides reached to 5.97%, which was 2.61 times compared with the extraction by water. Conclusion The optimized process parameters have the characteristics of the higher efficiency and the lower extracting temperature.更多还原