【摘要】 目的研究茶多酚等天然抗氧化剂对香烟烟雾致损的HEK293细胞的保护效能及JAK2/STAT3代谢调控机制。方法香烟过滤嘴中添加茶多酚、白芦藜醇、维生素C、芦丁、槲皮素、绿原酸、花青素、原花青素8种植物提取物后收集烟气,对HEK293细胞进行MTT对比研究及运用Western blot技术分析其Bcl-2、Bax、STAT3蛋白表达量变化。结果香烟烟雾可降低HEK293细胞存活率,呈浓度依赖关系;上调HEK293细胞中Bcl-2蛋白相对表达量,下调Bax、STAT3蛋白相对表达量,且各蛋白变化趋势呈浓度依赖效应;在过滤嘴中添加茶多酚、白芦藜醇、维生素C、芦丁、槲皮素、绿原酸、花青素、原花青素8种抗氧化剂明显抑制了香烟烟雾导致HEK293细胞死亡,结果具有显著性差异(P<0.05),其中以茶多酚效果最佳。结论 8种植物提取物均能对香烟所致HEK293细胞损伤产生一定的保护作用。更多还原

【Abstract】 Objective To investigate the metabolic regulation mechanism of tea polyphenols and other natural antioxidants antagonizing cigarette smoke damaging HEK293 cells and its JAK2/STAT3.Methods Eight kinds of antioxidants,tea polyphenols,resveratrol,vitamin C,rutin,quercetin,chlorogenicacid,anthocyanins and proanthocyanidins,were added to cigarette filters and the smoke was collected.HEK293 cells were studied by MTT trail data and the protein expression Bcl-2,Bax,and STAT3 were analyzed by Western blot technique.Results Cigarette smoke could reduce the HEK293 cell survival rates in a dose-dependent manner.With up-regulate Bcl-2 protein relative expression,down-regulate Bax,and STAT3 protein relative expression levels,each protein expression levels were in a dose-dependent effect for HEK293 cells.Comparing to the control,adding 8 kinds of antioxidants to cigarette filters could inhibit the increase of HEK293 cells death rates caused by cigarette smoke obviously.The results had significant differences(P<0.05),and tea polyphenols was the best.Conclusion Eight kinds of antioxidants can protect HEK293 cell from damage caused by cigarettes smoke.更多还原