【摘要】 为了优化沼气池产气效率和开展污泥微生物多样性研究,通过对PCR-DGGE条件的优化,建立了农村户用沼气池污泥微生物16S r DNA V3区DGGE分析技术,通过DGGE技术分析了沼气池污泥中细菌和古细菌微生物多样性随沼气池深度的变化规律。同时通过构建污泥样品mcr A功能基因克隆文库,应用RFLP技术,开展了农村户用沼气池污泥样品中与次级代谢产物相关基因的功能基因多样性研究。结果显示,农村户用沼气池污泥中含有丰富的微生物资源,其中细菌群落受污泥深度因素影响小,而浅层污泥中古细菌群落与深层污泥中古菌群落却存在明显差异。所采集的沼气池污泥中含有较为丰富的产甲烷菌资源,其中可能存在类似功能或产生类似代谢物质的产甲烷菌。该结果为进一步优化群落结构、筛选功能基因提供了科学依据。更多还原

【Abstract】 To optimize biogas production efficiency and investigate the microbial diversity of rural biogas tank sludge, the analysis technique of DGGE for 16 S r DNA V3 of microbe in the rural biogas tank was successfully established by the optimized PCR-DGGE. And then the bacterial and archaeal diversity with the change of sludge depth of biogas tank were studied using DGGE. At the same time, the mcr A gene library of sludge samples were constructed, based on this, the diversities of mcr A gene of rural biogas tank sludge were investigated using RFLP. The rural biogas tank sludge contains rich microbe, the sludge depth had less influence on the distribution of the bacterial community, while the archaea communities had obvious distinction with the change of the sludge depth. The results showed the biogas tank sludge had abundant methane producing bac-teria, which had similar functions or produce similar metabolic substances. These results provide a basis for further optimizing the community structure and screening functional genes of methane synthesis.更多还原