【摘要】 α-L-鼠李糖苷酶(α-L-rhamnosidase(EC 3.2.1.40))是一种水解酶,分布在GH13、GH28、GH78和GH106家族,其中3种GH78家族的α-L-鼠李糖苷酶具有典型的(α/α)6桶状结构,它能够特异性地水解许多糖苷类物质末端的α-L-鼠李糖基,在食品饮料加工工业和医药业中具有重要的应用价值。介绍了微生物来源的α-L-鼠李糖苷酶的性质及应用,主要阐述了α-L-鼠李糖苷酶基因的克隆、表达及该酶晶体结构方面的研究进展。更多还原

【Abstract】 α-L-rhamnosidase(EC 3.2.1.40) cleaves terminal α-L-rhamnose specifically from a large number of glycosides. The enzyme is a biotechnologically important hydrolytic enzyme due to its applications in the food and beverage processing industry and pharmaceutical industry. Based on primary sequence similarities, α-L-rhamnosidases are classified within the Carbohydrate-active enzymes(CAZy) database into four glycoside hydrolase(GH) families(GH13, GH28, GH78 and GH106 family), and three α-L-rhamnosidases of GH78 have a typical(α/α)6-barrel structure. Properties and applications of α-L-rhamnosidase from different microbial sources have been introduced. In addition, the recent developments on cloning, expression of α-L-rhamnosidase gene and the crystal structure of the enzyme were summarized.更多还原