血液高凝状态、血栓形成是导致溃疡性结肠炎(ulcerate colitis,UC)恶化的主要原因。本文旨在探讨蛋白C(protein C,PC)系统在UC小鼠中的变化及其可能的作用。(1)体内实验:采用饮用4%硫酸葡聚糖钠(dextran sulfate sodium,DSS)复制小鼠UC模型,造模后1周观察体重、结肠长度、脾重变化,并进行大体积分、组织学积分,免疫荧光法观察结肠平滑肌组织巨噬细胞数量,ELISA法测定血浆TNF-α、IL-6水平;活体荧光显微镜观察结肠黏膜微血管循环,免疫比浊法观察PC、蛋白S(protein S,PS)活性,免疫组化观察内皮细胞蛋白C受体(endothelial cell protein C receptor,EPCR)、血栓调节蛋白(thrombomodulin,TM)表达。(2)体外实验:分离小鼠结肠组织巨噬细胞,测定上清液中TNF-α、IL-6水平;分离、培养小鼠结肠黏膜微血管内皮细胞,分别以TNF-α、IL-6刺激后,检测其PC、PS、激活的蛋白C(activated protein C,APC)活性及EPCR、TM的表达。体内实验结果显示,与对照组相比,DSS组小...
【英文摘要】
Hypercoagulable state and thrombosis are major lethal causes of ulcerate colitis(UC). The aim of the present study is to explore the change and role of protein C(PC) system in UC thrombosis. 4% dextran sulfate sodium(DSS) was used to induce the UC model, and the body weight, the length of colon, and the weight of spleen were measured after intake of DSS as drinking water for 1 week. The macroscore and microscore were examined. The quantity of macrophage in colon smooth muscle was observed by immunofluoresce...
【基金】
supported by the National Natural Science Foundation of China(No.U1304802);
the Research Foundation of Henan University,China(No.2012YBZR020)
【更新日期】
2015-05-13
【分类号】
R574.62;R446.6
【正文快照】
This work was supported by the National Natural Science Foundation of China(No.U1304802)and the Research Foundation ofHenan University,China(No.2012YBZR020).**These authors contributed equally to this work.溃疡性结肠炎(ulcerative colitis,UC)是炎症性肠病(i
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