【摘要】 目的:研究白花丹醌对人肝星状细胞株(HSC-LX2)的作用,观察该药对细胞的增殖抑制率、细胞凋亡、分泌细胞外基质和金属蛋白酶功能的影响。方法:HSC-LX2与药物孵育48 h,以MTT法检测细胞增殖抑制率;以流式细胞术检测细胞凋亡情况;免疫组化法观察Ⅰ型、Ⅲ型胶原蛋白,MMP-1和MMP-13表达的部位和面积。结果:白花丹醌低、中、高浓度均可抑制HSC-LX2细胞增殖,诱导细胞发生凋亡,降低Ⅰ型和Ⅲ型胶原分泌水平,并增加其分泌MMP-1及MMP-13的能力。以上作用具有剂量依赖性,有统计学差异(P<0.05);中、高剂量组上述的作用比秋水仙碱强。结论:白花丹醌具有抑制HSC-LX2细胞增殖、诱导细胞发生凋亡、降低细胞外基质的分泌,并增加纤维蛋白降解酶分泌水平的能力,而对肝纤维化进程有干预作用;上述能力均有剂量依赖性;且中、高剂量组的干预能力强于秋水仙碱。更多还原

【Abstract】 This article was aimed to study the effects of plumbagin to human hepatic stellate cells. Observations were made on the influence of proliferation inhibition rate, apoptosis, secretion of extracellular matrix function and matrix metalloproteinase(MMP) by plumbagin. HSC-LX2 and drug were co-incubated for 48 hours. Then, MTT assay was used in the detection of inhibition of cell proliferation. The flow cytometry was used in the detection of apoptosis. Immunohistochemical method was used to observe type Ⅰ and Ⅲ collagen, MMP-1 and MMP-13 expression location and area. The results showed that the low, medium and high concentrations of plumbagin inhibited cell proliferation rate of HSC-LX2, induced apoptosis of cells, reduced the secretion of type Ⅰ and Ⅲ collagen, and increased the secretion ability of MMP-1 and MMP-13. Effects mentioned above were dose-dependent with statistical difference(P < 0.05). Effects in the medium and high concentrations groups were stronger than colchicine group. It was concluded that plumbagin had the ability to inhibit cell proliferation rate of HSC-LX2, induce apoptosis, reduce the secretion of extracellular matrix, and increase the secretion ability of fibrin degradation enzyme. Therefore, it had intervention effect on the process of liver fibrosis. All effects mentioned above were dose-dependent. And effects in the medium and high concentrations groups were stronger than colchicine group更多还原