【摘要】 目的:观察针刺对COPD模型大鼠的治疗效果及对外周血中HDAC2活性的影响。方法:连续3周对熏烟法建立COPD模型大鼠进行针刺治疗,仪器检测大鼠肺的通气能力。3周后抽取大鼠外周静脉血,用活性荧光分析技术测定其中HDAC2酶活性;用ELISA法检测外周血中IL-10、IL-8和TNF-α的表达含量。结果:针刺治疗的大鼠,与模型组的大鼠相比,肺通气水平明显上升(P<0.05),外周血的HDAC2活性亦明显上升(P<0.05),但是炎症因子IL-10、IL-8和TNF-α的表达含量明显降低(P<0.05)。结论:针刺治疗对于COPD模型大鼠具有疗效,结果也提示针刺疗效可能是通过调节HDAC2活性,从而消除炎症而起作用。更多还原

【Abstract】 This article was aimed to explore the effect of acupuncture on HDAC2 activity in peripheral blood of chronic obstructive pulmonary disease(COPD) rats. The COPD rat model was established by cigarette smoking. Acupuncture was given for 3 consecutive weeks. The lung ventilation of rat was detected by equipment. Peripheral blood of rat was taken after 3 weeks. The HDAC2 enzyme activity was detected by fluorescence technology. ELISA was used in the detection of IL-10, IL-8 and TNF-α expression in peripheral blood. The results showed that after acupuncture treatment, the lung ventilation was obviously increased compared with the model group(P < 0.05). Meanwhile, the HDAC2 activity in the peripheral blood of rat was obviously increased(P < 0.05). However, the expression of inflammatory cytokines IL-10, IL-8 and TNF-α was obviously reduced(P < 0.05). It was concluded that acupuncture treatment had a good efficacy on COPD rat. It indicated that acupuncture treatment might regulate HDAC2 activity to reduce inflammation.更多还原