【摘要】 目的建立同时测定板蓝根颗粒中腺苷、尿苷、鸟苷、(R,S)-告依春的一测多评法。方法以腺苷为内标物,确定板蓝根中其他3种成分相对于腺苷的校正因子,通过相对校正因子(relative correction factor,RCF)对尿苷、鸟苷、(R,S)-告依春进行定量,实现一测多评(计算法);同时采用外标法测定板蓝根颗粒中4种成分的含量(实测值),并比较一测多评法与外标法测定结果的差异。结果各成分相对校正因子重现性良好。尿苷、鸟苷、(R,S)-告依春按一测多评方法与外标法测定结果差异无统计学意义。结论本研究建立的一测多评法简便,测定结果准确可靠,可为板蓝根颗粒的质量控制提供参考。更多还原

【Abstract】 Objective To establish a quantitative analysis of multi-components by single-marker( QAMS) for determination of four components( adenosine,uridine,guanosine,( R,S)-goitrin) in Isatidis Radix granules. Method The QAMS for Isatidis Radix granules was established and validated and adenosine was selected as the internal reference substance. The relative correction factor( RCF) of other three components were calculated. The contents of four components were determined by both external standard method and QAMS. The QAMS method was validated through comparison of the results obtained by the two different methods. Results The RCFs of uridine,guanosine and( R,S)-goitrin all showed good reproducibility within certain ranges. For Isatidis Radix granules,uridine,guanosine and( R,S)-goitrin,there was no significant difference between the quantitative results of the two methods. Conclusions The QAMS method is simple,feasible and credible to be used for comprehensive quality control of Isatidis Radix granules.更多还原