【摘要】 目的建立高效空间排阻色谱(high performance size exclusion chromatography,HPSEC)分析方法,测定肝龙胶囊中分子量分布并采用Lowry法测定其总肽含量。方法采用superdex peptide 10/300 GL(10 mm×300 mm)色谱柱,以p H=6.0、0.05 mol/L的磷酸盐缓冲液为流动相,其中含有0.1 mol/L的Na Cl;流速为0.7 m L/min;柱温25℃;检测波长214 nm。结果总肽含量测定的线性范围为0.08~0.4 mg,r=0.9996;含量精密度试验的RSD为0%(n=6),重复性试验的RSD为1.1%(n=6)。标准物质分子量的回归方程为log Mr=5.1455-0.0871tR,r=0.9983,分子量的线性范围为2.68×102~5.73×103Da,分子量精密度试验RCD为0.08%,重复性试验RSD为1.3%。结论该方法准确、简便、快速,可作为肝龙胶囊的质量控制方法。更多还原

【Abstract】 Objective To establish the molecular weight distribution of Ganlong capsule by HPSEC the content of the peptide determined by Lowry and Methods The superdex peptide 10 /300 GL(10 mm × 300 mm) column was used.The p H = 6.0 and phosphate buffer of 0.05 mol / L was used as the mobile phase,containing 0.1 mol / L Na Cl.The flow rate was set at 0.7 m L / min; The column temperature was 25 ℃; The detection wavelength was 214 nm.Results The content of the peptide ranged from 0.08 mg to 0.4 mg(r = 0.9996).The RSDs of measurement precision of molecular weight and content were 0.08% and 0%(n = 6),respectively.The RSDs of the repeatability were 1.3% and 1.1%(n = 6); The regression equation of standard material was log Mr = 5.1455-0.0871 tR,r = 0.9983,the relative molecular weight ranged from 2.68 × 102 Da ~ 5.73 × 103Da(r = 0.9983).Conclusion on The method is simple and rapid for determining the peptide content and the molecular weight distribution of Ganlong capsule.It can be used quality control method for Ganlong capsule.更多还原