【摘要】 目的研究股骨头坏死(osteonecrosis of the femeral head,ONFH)患者滑膜组织来源的类成纤维细胞对人Burkitt淋巴瘤(Burkitt lymphoma,BL)Raji细胞株增殖的影响。方法首先分离和培养来自ONFH患者滑膜组织的原代类成纤维细胞,随后将其与Raji细胞共培养;用细胞计数试剂盒(CCK-8)检测Raji细胞的增殖情况;用实时定量PCR(quantitative real-time PCR,qRT-PCR)检测Raji细胞中P53、P21及CD9基因的表达水平。结果 ONFH滑膜来源的细胞为贴壁的类成纤维细胞。共培养组中的Raji细胞增殖速率显著高于单独培养组(只培养Raji细胞)。相对于单独培养组,共培养24h后,P53、P21及CD9的表达量分别为0.82(P<0.05)、1.75(P<0.05)及1.99(P<0.05),48h后,则分别为0.72(P<0.05)、1.03及0.56(P<0.05)。结论在共培养条件下,ONFH患者滑膜组织来源的类成纤维细胞可能通过调控Raji细胞中P53、P21及CD9的表达来促进Raji细胞的增殖。更多还原

【Abstract】 Objective To investigate the effect of fibroblast-like cells which derived from the synovial membrane of the patients with osteonecrosis of the femeral head(ONFH)on the proliferation of Raji cells from human Burkitt lymphoma(BL). Methods Primary fibroblast-like cells derived from the synovial membrane of ONFH patients were isolated and cultured,and then a co-culture system was established with Raji cells.The proliferation of Raji cells was determined by CCK-8 assay,and the expressions of P53,P21 and CD9mRNA in Raji cells were determined by quantitative real-time PCR(qRT-PCR).Results The cells derived from the synovial membrane of ONFH were adherent fibroblast-like cells.The proliferation rate of Raji cells in co-culture group was significantly higher than that in individual group(cultured Raji cells alone).Compared with the individual group,the relative expression amount of P53,P21,and CD9 mRNA after co-cultured for 24 hours in co-culture group was 0.82(P<0.05),1.75(P<0.05),and 1.99(P<0.05),respectively;and was 0.72(P<0.05),1.03,and 0.56(P<0.05),respectively after co-cultured for 48 hours. Conclusions Under co-culture condition,the fibroblast-like cells from synovial membrane of ONFH patients could significantly promote the proliferation of Raji cells by regulating the expression of P53,P21,and CD9 mRNA.更多还原