【摘要】 目的 探讨高脂膳食和8周有氧耐力运动对大鼠腓肠肌腺苷酸活化蛋白激酶(AMPK)/乙酰辅酶A羧化酶(ACC)信号通路和膜蛋白脂肪酸转位酶CD36含量的影响。方法 建立营养性肥胖大鼠模型，并随机分为肥胖安静组(OC组)和肥胖运动组(OE组)，另设普通饲料安静组(NC组)和普通饲料运动组(NE组)。运动干预结束后检测腓肠肌AMPKα、p-AMPKα、ACC、p-ACC和膜蛋白CD36的蛋白水平。结果 1) NE组与NC、OE及OC组相比，p-AMPKα的蛋白水平显著升高(P<0.01); OC组p-AMPKα的蛋白水平显著低于NC组(P<0.01)。2) OC组p-ACC蛋白水平显著低于NC组(P<0.01); OE组p-ACC的蛋白水平显著高于OC组(P<0.01)。3) NE组与NC、OE及OC组相比，OC组与NC组相比，膜蛋白CD36含量均无显著性变化(P>0.05)。结论 1)运动可改善高脂膳食引起的AMPK/ACC信号通路障碍。2)运动对体质量不同大鼠p-ACC蛋白水平的影响存在差异。3)腓肠肌膜蛋白CD36的含量并没有伴随AMPK/ACC信号通路的激活或抑制而发生显著变化。更多还原

【Abstract】 Objective To investigate the effects of high-fat diet and 8 weeks aerobic endurance exercise(treadmill exercise) on AMP-activated protein kinase(AMPK)/acetyl-CoA carboxylase(ACC)signaling pathway and fatty acid translocase CD36 protein content in rat gastrocnemius muscles.Methods A rat model of nutritional obesity was established by feeding rats a high-fat diet, and then the obese rats were randomly divided into obese control group(OC group) and obese exercise group(OE group); In addition, a normal diet control group(NC group) and a normal diet exercise group(NE group)were set up. The exercise groups(OE and NE groups) were arranged by aerobic endurance exercise for8 weeks. After the experiment, the levels of AMPKα, p-AMPKα, ACC, p-ACC and membrane protein CD36 in all groups were detected by Western blotting in gastrocnemius muscles. Results 1) Compared with the NC group, p-AMPKα protein levels had a significant increase in the NE group(P<0.01). p-AMPKαprotein levels in the OE group were significantly higher than that in the OC group(P<0.01). p-AMPKαprotein levels in the OC group were significantly lower than that in the NC group(P<0.01). 2) Compared with the NC group, p-ACC protein level in the OC group were significantly decreased(P<0.01). p-ACC protein levels had a significant increase in the OE group as compared with the OC group(P<0.01).3) Compared between the NE group and NC group, the OE group and OC group, and the OC group and NC group, the content of membrane protein CD36 showed no significant change(P>0.05). Conclusion 1) The treadmill exercise could improve AMPK/ACC signaling pathway. 2) The impacts of exercise on p-ACC levels were different in rats with different body mass. 3) The membrane protein CD36 content in the rat gastrocnemius muscles did not significantly change whether AMPK/ACC signaling pathway was activated or inhibited.更多还原