【摘要】 目的 研究dl-扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜的刺激性。方法 将9只雌性小型猪按体质量随机分为空白胶组、dl-扁桃酸阴道热敏凝胶剂组和市售壬苯醇醚凝胶(N-9凝胶)组,肌肉注射苯甲酸雌二醇400μg/kg(促发情), 12 d后建立小型猪阴道刺激性试验模型，阴道给予5 mL凝胶,每日一次,连续给药7 d,在给药前和末次给药后的24 h与72 h，收集阴道灌流(CVL)液,流式细胞仪计数炎性细胞和中性粒细胞数目;末次给药72 h后处死小型猪,取阴道组织,观察阴道黏膜组织病理学改变。结果 dl-扁桃酸阴道热敏凝胶剂组CVL液中炎性细胞、中性粒细胞数目明显低于N-9凝胶组(P<0.05)。小型猪阴道黏膜组织HE染色显示, dl-扁桃酸阴道热敏凝胶剂处理组阴道黏膜无明显上皮破损、血管充血以及炎症细胞浸润,其刺激性评分(5.10±0.47),与空白胶组(3.30±1.60)比较无显著性差异(P>0.05)，明显低于N-9凝胶组(20.50±3.50)(P<0.05)。结论 dl-扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜刺激性较小。更多还原

【Abstract】 Objective To study the vaginal irritation of dl-Mandelic acid thermosensitive in situ Gel(dl-Mandelic acid-TISG) in miniature pigs. Methods Nine miniature pigs injected with estradiol benzoate(400 μg/kg) were randomly divided into three groups: control group, dl-Mandelic acid-TISG group and nonoxynol-9 gel(N-9 gel) group. Each miniature pig was administrated with 5 mL gel through vagina once daily for 7 days. Cervicovaginal lavage(CVL) fluids were collected before administration and at the 24 h and 72 h after the last administration. The number of inflammatory cells and neutrophilic granulocytes in the CVL were detected by the flow cytometry. The miniature pigs were sacrificed at 72 h after the last administration. Histopathological changes of the vaginal mucosa were observed under light microscope. Results The number of inflammatory cells and neutrophilic granulocytes in the dlMandelic acid-TISG group were significantly lower than that in the N-9 group(P<0.05). Histological scoring of vaginal mucosa showed that there were no obvious mucosal epithelium damage, vascular congestion and inflammatory cell infiltration(5.10 ± 0.47) in the dl-Mandelic acid-TISG group.There was no significant difference(3.30 ± 1.60, P>0.05) compared with the control group(P>0.05) but significant difference compared with the N-9 group(20.50 ± 3.50, P<0.05). Conclusion The vaginal irritation of dl-Mandelic acid-TISG in miniature pigs is mild.更多还原