【摘要】 目的蜜炙前后桑白皮对经方泻白散止咳平喘作用的影响。方法 KM小鼠随机分为模型对照组、可待因(4.68 mg·kg-1)组、泻白散生品和蜜炙0.57、1.14、2.28、4.55、9.10、18.20 g·kg-1剂量组,连续10 d灌胃给药,采用浓氨水引咳实验检测引咳潜伏期、2 min内咳嗽次数;Wistar大鼠随机分为空白对照组、模型对照组、地塞米松(0.000 4 g·kg-1)组、泻白散生品和蜜炙3.15、9.45 g·kg-1剂量组,造模大鼠于第1、7天腹腔注射卵蛋白混悬液1 m L致敏。第15天起,每天激发造模,共5 d。第20天,记录大鼠10 min内出现Ⅱ级哮喘的潜伏期,采用酶联免疫吸附法测定血清中脂质过氧化物(LPO)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的含量。结果泻白散含生品桑白皮4.55、9.10、18.20 g·kg-1剂量组、含蜜炙桑白皮各剂量组均可显著延长咳嗽潜伏期,减少咳嗽次数。泻白散生品、蜜炙高剂量组均显著降低哮喘大鼠血清脂质过氧化物(LPO)、白细胞介素-4(IL-4)含量,升高了血清干扰素-γ(IFN-γ)。结论蜜炙组较生品组作用效果更好。提示清除细胞毒性过氧化物,调节免疫,增强抗病毒能力可能是泻白散平喘作用的药理学机理。更多还原

【Abstract】 Objective To investigate the relieving cough and asthma activities of Cortex Mori before and after hwey processing. Methods KM mice were randomly divided into model control group,positive control group,codeine( 4. 68 mg·kg- 1)group,the crude and hwey processed Cortex Mori 0. 39,0. 78,1. 56,3. 12,6. 24 g·kg- 1group,giving drugs 10 days. The model of cough induced by concentrated ammonia water was used to observe the cough latent phase and times in 2 min. Wistar rats were randomly divided into blank group,model control group,dexamethasone( 0. 000 4 g·kg- 1) group,the crude and hwey processed Cortex Mori 3. 15,9. 45 g·kg- 1group. Model rats were allergized by injecting egg albumin for intraperitoneal injection at 1st and 7th day. From 15 th day,to stimulate making model,all 5 days. The incubation periods of Ⅱgrade asthmatic within 10 min in rats were recorded. The contents of LPO,IL- 4 and IFN- γ in serum were detected by enzyme linked immunosorbent assay. Results Compared with the model group,the latent phase was significantly longer and the cough times per two minutes was significantly reduced in Xiebaisan with crude Cortex Mori 4. 55,9. 10,18. 20 g·kg- 1and hwey processed Cortex Mori groups. The contents of LPO,IL- 4 in serum of crude Xiebaisan and hwey processed Cortex Mori high dose groups were significantly reduced,and the contents of IFN- γ were increased. Conclusion The results of hwey processed groups were better than crude groups. The results prompted that cleaning cytotoxicity peroxides,adjusting immunization and reinforcing anti- virus capabilities may be the pharmacology mechanism of relieving cough and asthma activities.更多还原