【摘要】 采用酶抑制法测定了10种氨基甲酸酯类农药的检出限,并进行了酶活性的减退试验,结果表明,涕灭威亚砜的检出限较低,速灭威、灭多威、异丙威、涕灭威、甲萘威、涕灭威砜、仲丁威的检出限相对较高,克百威和3-羟基克百威的检出限更高;配制好的酶在9℃的冰箱中保存,其活性并不随着时间的推移而降低,但是每天测试的酶的活性都有一定的差别;与酶、显色剂和底物反应的提取液的浓度影响酶抑制法测定农残的结果。更多还原

【Abstract】 The detection limits of 10 carbamate pesticides were determined on enzyme inhibition mothod; and the impairment test of enzyme activity were done. The results showed : The detection limits of Aldicarb- sulfoxide was low;and the detection limits of metacrate 、methomyl、mipcide、aldicarb、carbaryl、aldicarb- sulfone、enobucarb were a little higher,the detection limits of carbofuran and 3- hydroxycarbofuran were higeter. The enzyme which has been made up were stored in refrigerator witch temperature was 9℃,Its activity didn,t reduce as time went by,every test of activity of the enzyme had certain difference; the repeatability was bad on the determination of pesticide by enzyme inhibition method. The determination result was influenced by the concentration of the extract which reactivities with the enzyme 、chromogenic agent and substrate.更多还原