【摘要】 建立复方扶芳藤合剂中人参皂苷Rb1的含量测定方法。利用高效液相色谱法,采用Ulitimate XB-C18(2)柱(250 mm×4.6 mm,5μm);流动相:V(乙腈)-V(水)(30∶70);柱温:室温;流速:0.7 m L·min-1;检测波长:203 nm;进样量:10μL。人参皂苷Rb1的线性回归方程为Y=816 921.4 X-689.2,r=0.999 9。人参皂苷Rb1在0.01~1μg范围内呈良好的线性关系。人参皂苷Rb1的平均回收率为99.08%,RSD为1.33%(n=6)。该方法稳定、重复性好,结果准确,可作为复方扶芳藤合剂中人参皂苷Rb1的含量测定方法。更多还原

【Abstract】 To establish a method to determine the content of ginsenoside Rb1 in compound Fufangteng mixture.HPLC was used for the determination of ginsenoside Rb1 in compound Fufangteng mixture.An Ulitimate XB-C18(2) column(250 mm × 4.6 mm,5 μm) with the mobile phase consisting of acetonitrile-water(30 ∶ 70) and the flow rate of 0.7 m L·min-1at room temperature were used.The detection wavelength was set at 203 nm.The injection volume was 10 μL.Ginsenoside Rb1 showed a good linear relationship at the range of 0.01 to 1 μg,r = 0.999 9.And the average recoveries of ginsenoside Rb1 were 99.08%,RSD were 1.33%.The method is steady and with good repeatability,and can be used to determine the content of ginsenoside Rb1 in compound Fufangteng mixture.更多还原