【摘要】 从川西南不同区域的酒曲中分离纯化出18株产酯酵母菌株,通过测量透明圈直径初筛、发酵产酯能力和四大酯催化生成能力复筛获得优良的产酯酵母,并考察其对酒精、温度和酸度的耐受性。结果表明:酵母Y2的产酯能力最好,其在高粱发酵培养基中总酯产量为3.273 g/L,在100 m L的酯化体系中催化生成乙酸乙酯和己酸乙酯的量为2.731 g/L和1.921 g/L,该菌株能够在酒精浓度10%、p H2.0、温度为38℃的条件下生长,具有良好的耐酒精、耐酸和耐温能力。更多还原

【Abstract】 18 strains of ester producing yeast were separated and purified in liquor making koji from the different zones of southwest of Sichuan,and an excellent ester-producing yeast strain was screened through the transparent circle diameter measuring and ability of ester-producing fermentation and catalytic production of four kinds of esters. Its tolerance to alcohol,temperature and acidity were studied. The results showed that ability of ester producing of yeast strain Y2 was the highest. Its total ester yield in the sorghum fermentation medium was 3. 273 g / L,and the catalytic production yield of ethyl acetate and ethyl hexanoate were 2. 731 g / L and 1. 921 g / L in 100 m L esterification system. The yeast strain with good tolerance to alcohol,acid and heat could be grown under the conditions of alcohol concentration 10%,p H2. 0,temperature 38℃.更多还原