【摘要】 根据GenBank中公布的脑心肌炎病毒(Encephalomyocarditis virus EMCV)3D基因保守区段设计并合成1对引物,以提取的EMCV核酸为模板,分别进行荧光定量RT-PCR扩增,优化反应体系及条件,建立脑心肌炎病毒荧光定量RT-PCR检测方法,并初步运用于静注人免疫球蛋白(IVIG)纳米膜过滤工艺去除EMCV效果的验证.结果显示该方法实验内和实验间的精密度均小于5%,最低检测限为102copies/μL;纳米膜过滤工艺可使样品中的EMCV滴度下降4Logs.更多还原

【Abstract】 Based on the conservative region of 3D gene of Encephalomyocarditis virus(EMCV)published in GenBank,apair of primers were Designed and synthesized.Use the extracted EMCV nucleic acid as a template for fluorescence quantitative RT-PCR amplification,optimization of reaction system and conditions.Establishencephalomyocarditis virus(EMCV)fluorescence quantitative RT-PCR detection method,and applied to static note human immunoglobulin(IVIG)nano membrane filtration process to remove EMCV effect validation.The results showed both of the precision of intra and inter assay was<5% in Ct,and the lowest detective limit was 102 copies/μL.Nano membrane filtration process can make the samples of EMCV drops fell 4 logs.更多还原