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中国荷斯坦牛乳腺中β-酪蛋白的变化研究
Study on the change of β-casein in Chinese holstein mammary gland
【摘要】 对乳腺中β-酪蛋白在蛋白水平和基因水平的表达研究进行对比,系统阐述中国荷斯坦牛乳腺中β-酪蛋白的变化规律。结果表明:中国荷斯坦牛乳腺发育过程中,β-酪蛋白出现在妊娠中期,表达水平逐渐升高,至泌乳期达到最高,退化期逐渐下降。β-酪蛋白基因表达水平在青春期很低,到妊娠期迅速升高。整个泌乳期β-酪蛋白表达水平比较平稳,各时点无差异,而其基因表达水平却有显著差异。退化早期,β-酪蛋白及其基因表达水平开始逐渐下降,但仍与青春期有显著差异。
【Abstract】 In the study, theβ-casein of Chinese Holstein dairy cow mammary gland on the protein level and gene level were compared and the changing regular ofβ-casein were systematically expounded.The results showed thatβ-casein appeared mid-pregnancy during mammary growing, advanced gradually and reached the peak in lactation, and decreased step by step in involution. The expression ofβ-casein gene was very low in puberty, increased fast after early pregnancy. The level ofβ-casein was stable during lactation, and there was no difference among different time, however, the expression ofβ-casein gene had significant difference. The expression ofβ-casein and its gene beginned to descend gradually in early involution, however which had significant difference than that of puberty.
【Key words】 Chinese Holstein dairy cow; mammary gland; laser scanning confocal microtechnique; β-casein;
- 【文献出处】 中国乳品工业 ,China Dairy Industry , 编辑部邮箱 ,2015年04期
- 【分类号】S823
- 【被引频次】2
- 【下载频次】112