Tissue culture and rapid propagation of Taraxacum kok-saghyz Rodin were conducted with root as the explant. The results showed that 500mg/L of activate carbon in the MS medium of pre-culture tested three days could significantly inhibit the browning of explants. The best culture medium(MS+0.6 mg/L 6-BA+0.3 mg/L 2,4-D)was suitable for callus induction of root, and the average induction rate was 90.0%. The differentiation medium was MS+0.8 mg/L 6-BA +0.3 mg/L NAA. The rooting medium was 1/2MS+0.2 mg/L NAA.